make.lookup
The make.lookup command allows you to create custom lookup files for the shhh.flows command. To run this tutorial please download: makelookup.zip.
Default Options
The reference, flow and error parameters are required.
mothur > make.lookup(reference=HMP_MOCK.v53.fasta, flow=H3YD4Z101.mock3.flow_450.flow, error=H3YD4Z101.mock3.flow_450.error.summary
Options
barcode
The barcode parameter is used to provide the barcode sequence. Default=AACCGTGTC.
key
The key parameter is used to provide the key sequence. Default=TCAG.
order
The order parameter is used to select the flow order. Options are A, B and I. Default=A, meaning flow order of TACG.
threshold
The threshold parameter is.… Default=10000.
Revisions
- 1.31.0 - First Introduced