Throughout 2020, Pat will offer workshops on mothur and programming in R for microbial ecologists. These will be offered online. If you are interested in attending and would like to register or get more information about fees and specifics, please email him his gmail account (userid: pdschloss). Other workshops will be announced throughout the year.
- March 30-April 1: mothur
This workshop is geared towards people with diverse backgrounds so you shouldn’t feel like this isn’t for you if you are a beginner. I assume no previous computer programming experience. The workshop is an even blend of lecture/discussion and hands on use of mothur with real sequence data. Here is a rough outline of what the workshop schedule will look like (although it is subject to some minor changes)...
’ Day 1:’
- Overview of methods used in microbial ecology
- DNA sequencing technologies (Sanger, 454, Illumina, IonTorrent, PacBio)
- Introduction to mothur, command line interface, and GUI
- Improving sequence quality
- Chimera detection
- Distance calaculations
- Amplicon sequence variants
- OTU identification
- Sequence/OTU classification
- Intro to microbial ecology
- Methods of measuring alpha and beta diversity
- Population level analyses
This workshop is being filled on a first come, first served basis. The workshop is geared towards people with interests in microbial ecology that would like to learn R or to learn it better so you shouldn’t feel like this isn’t for you if you are a beginner. I assume no previous computer programming experience. The workshop is an even blend of lecture/discussion and hands on use of R with real data and typically uses mothur output files as a starting point. It is taught around the generation of data visualization methods commonly used by microbial ecologists. Along the way we’ll learn a number of ways to work with data from within R. Here is a rough outline of what the workshop schedule will look like (it is subject to some minor changes)...
- Stripcharts and box and whisker plots
- Line plots
- Subsetting data
- Reading and writing data
- Basic parametric and non-parametric analyses
The workshop will give you a pretty thorough set of working examples for how to use packages like readr, dplyr, and ggplot2 with data from mothur; however, prior knowledge of mothur is not a prerequisite.