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Group file

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The group file is used to assign sequences to a specific group. It consists of 2 columns separated by a tab. The first column contains the sequence name. The second column contains the group name. Here is an example:

AY457915	A	
AY457914	A	
AY457913	A	
AY457912	A	
AY457911	A	
AY457910	A	
AY457909	A	
AY457908	A	
AY457907	A	
AY457906	A	


  1. Be sure not to include the ">" in the name of the sequence.
  2. You sequence name and group name cannot contain blank spaces. For example, the following will cause an error: AY457915 Dirt Sample
  3. DO NOT use a hyphen in group names. The "-" character is used within mothur to separate group names, labels, taxonomies, ect. Including a hyphen will cause issues in your downstream analysis.