make.fastq

The make.fastq command reads a fasta file and a quality file and creates a fastq file.

Default settings

The make.fastq command parameters are fasta and qfile. Both are required.

mothur > make.fastq(fasta=stool.fasta, qfile=stool.qual)

Options

format

The format parameter is used to indicate whether your sequences are sanger, solexa, illumina1.8+ or illumina, default=sanger.

Revisions

  • 1.29.0 Added format parameter.
  • 1.30.0 Added illumina1.8+ format.